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From the Publisher:
This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. This third edition contains 70% new material with 165 new articles covering classical as well as rapidly emerging technologies. Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for further ease of use. This collection enables researchers at all stages of their careers to embark on basic biological problems using a variety of technologies and model systems.
KEY FEATURES:
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Indispensable Bench Companion for every Life Science Laboratory
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Presents Easy-To-Use Protocols in a Step-By-Step, Reader Friendly Format
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Provides the Latest Information on the Plethora of Technologies Needed to Tackle Complex Biological Problems
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Includes Numerous Illustrations, Some in Full Color, Supporting Steps and Results
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Written by Leading Researchers Worldwide Who Have First-Hand Expertise in the Techniques
Table of Contents:
CONTENTS OF VOLUME 1
PART A. Cell and Tissue Culture: Associated Techniques
Section 1: General Techniques
Chapter 1. Setting up a Cell Culture Laboratory
Chapter 2. General Procedures for Cell Culture
Chapter 3. Counting Cells
Chapter 4. Cell Proliferation Assays: Improved Homogeneous Methods Used to Measure the Number of Cells in Culture
Chapter 5. Development of Serum-Free Media: Optimization of Nutrient Composition and Delivery Format
Chapter 6. Cell Line Authentication
Chapter 7. Detection of Microbial and Viral Contaminants in Cell Lines
Section 2: Culture of Specific Cell Types: Stem Cells
Chapter 8. Neural Crest Stem Cells
Chapter 9. Postnatal Skeletal Stem Cells: Methods for Isolation and Analysis of Bone Marrow Stromal Cells from Postnatal Murine and Human Marrow.
Chapter 10. Establishment of Embryonic Stem Cell Lines from Adult Somatic Cells by Nuclear Transfer
Chapter 11. T-Cell Isolation and Propagation in vitro
Chapter 12. Generation of Human and Murine Dendritic Cells
Section 3: Culture of Specific Cell Types: Hemopoietic, Mesenchymal, and Epithelial
Chapter 13. Clonal Cultures in vitro for Hemopoietic Cells Using Semisolid Agar Medium
Chapter 14. Human Skeletal Myocytes
Chapter 15. Growing Madin-Darby Canine Kidney Cells for Studying Epithelial Cell Biology
Chapter 16. Cultivation and Retroviral Infection of Human Epidermal Keratinocytes
Chapter 17. Three-Dimensional Cultures of Normal and Malignant Human Breast Epithelial Cells to Acheive in vivo-like Architecture and Function
Chapter 18. Primary Culture of Drosophilia Embryo Cells
Chapter 19. Laboratory Cultivation of Caenorhabditis elegans and Other Free-Living Nematodes
Section 4: Differentiation and Reprogramming of Somatic Cells
Chapter 20. Induction of Differentiation and Cellular Manipulation of Human Myeloid HL-60 Leukemia Cells
Chapter 21. Cultured PC12 Cells: A Model for Neuronal Function, Differentiation, and Survival
Chapter 22. Differentiation of Pancreatic Cells into Hepatocytes
Chapter 23. TERA2 and Is NTERA2 Subline: Pluripotent Human Embryonal Carcinoma Cells
Chapter 24. Embryonic Explants from Xenopus laevis as an Assay System to Study Differentiation of Multipotent Precursor Cells
Chapter 25. Electofusion: Nuclear Reprogramming of Somatic Cells by Cell Hybridization with Pluripotential Stem Cells
Chapter 26. Reprogramming Somatic Nuclei and Cells with Cell Extracts
Section 5: Immortalization
Chapter 27. Immortalization of Primary Human Cells with Telomerase
Chapter 28. Preparation and Immortalization of Primary Murine Cells
Section 6: Somatic Cell Hybrids
Chapter 29. Viable Hybrids between Adherent Cells: Generation, Yield Improvement, and Analysis
Section 7: Cell Separation Techniques
Chapter 30. Separation and Expansion of Human T Cells
Chapter 31. Separation of Cell Populations Synchronized in Cell Cycle Phase by Centrifugal Elutriation
Chapter 32. Polychromatic Flow Cytometry
Chapter 33. High-Speed Cell Sorting
Section 8: Cell Cycle Analysis
Chapter 34. Cell Cycle Analysis by Flow and Laser-Scanning Cytometry
Chapter 35. Detection of Cell Cycle Stage in situ in Growing Cell Populations
Chapter 36. In vivo DNA Replication Labeling
Chapter 37. Live Cell DNA Labeling and Multiphoton/ Confocal Microscopy
Section 9: Cytotoxic and Cell Growth Assays
Chapter 38. Cytotoxicity and Cell Growth Assays
Chapter 39. Micronuclei and Comet Assay
Section 10: Apoptosis
Chapter 40. Methods in Apoptosis
Section 11: Assays of Cell Transformation, Tumorigenesis, Invasion, and Wound Healing
Chapter 41. Cellular Assays of Oncogene Transformation
Chapter 42. Assays of Tumorigenicity in Nude Mice
Chapter 43. Transfilter Cell Invasion Assays
Chapter 44. Endothelial Cell Invasion Assays
Chapter 45. Analysis of Tumor Cell Invasion in Organotypic Brain Slices Using Confocal Laser-Scanning Microscopy
Chapter 46. Angiogenesis Assays
Chapter 47. Three-Dimensional, Quantitative, in vitro Assays of Wound Healing Behavior
Section 12: Electrophysiological Methods
Chapter 48. Patch Clamping
Section 13: Organ Cultures
Chapter 49. Preparation of Organotypic Hippocampal Slice Cultures
Chapter 50. Thyroid Tissue-Organotypic Culture Using a New Approach for Overcoming the Disadvantages of Conventional Organ Culture
PART B. Viruses
Section 14: Growth and Purification of Viruses
Chapter 51. Growth of Smliki Forest Virus
Chapter 52. Design and Production of Human Immunodeficiency Virus-Derived Vectors
Chapter 53. Construction and Preparation of Human Adenovirus Vectors
Chapter 54. Production and Quality Control of High-Capacity Adenoviral Vectors
Chapter 55. Novel Approaches for Production of Recombinant Adeno-Associated Virus
PART C. Antibodies
Section 15: Production and Purification of Antibodies
Chapter 56. Production of Peptide Antibodies in Rabbits and Purification of Immunoglobulin
Chapter 57. Preparation of Monoclonal Antibodies
Chapter 58. Rapid Development of Monoclonal Antibodies Using Repetitive Immunizations, Multiple Sites
Chapter 59. Phage-Displayed Antibody Libraries
Chapter 60. Ribosome Display: In vitro Selection of Protein-Protein Interactions
Chapter 61. Epitope Mapping by Mass Spectrometry
Chapter 62. Mapping and Characterization of Protein Epitopes by Using the SPOT Method
Chapter 63. Determination of Antibody Specificity by Western Blotting
Chapter 64. Enzyme-Linked Immunoabsorbent Assay
Chapter 65. Radioiodination of Antibodies
PART D. Immunocytochemistry
Chapter 66. Immunofluorescence Microscopy of Cultured Cells
Chapter 67. Immunofluorescence Microscopy of the Cytoskeleton: Combination with Green Fluorescent Protein Tags
Chapter 68. Immunocytochemistry of Frozen and of Paraffin Tissue Sections
PART E. APPENDIX
Chapter 69. Representative Cultured Cell Lines and Their Characteristics
CONTENTS OF VOLUME 2
PART A. Organelles and Cellular Structures
Section 1: Isolation: Plasma Membrane, Organelles, and Cellular Structures
Chapter 1. Detergent-Resistant Membranes and the Use of Cholesterol Depletion
Chapter 2. Isolation and Subfractionation of Plasma Membranes to Purify Caveolae Separately from Lipid Rafts
Chapter 3. Immunoisolation of Organelles Using Magnetic Solid Supports
Chapter 4. Purification of Rat Liver Golgi Stacks
Chapter 5. Isolation of Rough and Smooth Membrane Domains of the Endoplasmic Reticulum from Rat Liver
Chapter 6. Purification of COPI Vesicles
Chapter 7. Purification of Clathrin-Coated Vesicles from Bovine Brain, Liver, and Adrenal Gland
Chapter 8. Isolation of Latex Bead-and Mycobacterial-Containing Phagosomes
Chapter 9. Isolation of Peroxisomes
Chapter 10. Isolation of Mitochondria from Mammalian Tissues and Cultured Cells
Chapter 11. Subcellular Fractionation Procedures and Metabolic Labeling Using [35S] Sulfate to Isolate Dense Core Secretory Granules from Neuroendocrine Cell Lines
Chapter 12. Preparation of Synaptic Vesicles from Mammalian Brain
Chapter 13. Preparation of Proteasomes
Chapter 14. Preparation of Cilia from Human Airway Epithelial Cells
Chapter 15. Isolation of Nucleoli
Chapter 16. Purification of Intermediate-Containing Spliceosomes
Chapter 17. Isolation of Cajal Bodies
Chapter 18. Replication Clusters: Labeling Strategies for the Analysis of Chromosome Architecture and Chromatin Dynamics
Chapter 19. Isolation of Chromosomes for Flow Analysis and Sorting
Section 2: Vital Staining of Cells/ Organelles
Chapter 20. Vital Staining of Cells with Fluorescent Lipids
Chapter 21. Labeling of Endocytotic Vesicles Using Fluorescent Probes for Fluid-Phase Endocytosis
Section 3: Protein Purification
Chapter 22. Preparation of Tubulin from Porcine Brain
Chapter 23. Purification of Smooth Muscle Actin
Chapter 24. Purification of Nonmuscle Actin
Chapter 25. Purification of Skeletal Muscle Actin
PART B. Assays
Section 4: Endocytic and Exocytic Pathways
Chapter 26. Permeabilized Epithelial Cells to Study Exocytic Membrane Transport
Chapter 27. Studying Exit and Surface Delivery of Post-Golgi Transport Intermediates Using in vitro and Live-Cell Microscopy-Based Approaches
Chapter 28. Assays Measuring Membrane Transport in the Endocytic Pathway
Chapter 29. Microsome-Based Assay for Analysis of Endoplasmic Reticulum to Golgi Transport in Mammalian Cells
Chapter 30. Cotranslational Translocation of Proteins into Microsomes Derived from the Rough Endoplasmic Reticulum of Mammalian Cells
Chapter 31. Use of Permeabilsed Mast Cells to Analyze Regulated Exocytosis
Section 5: Membranes
Chapter 32. Syringe Loading: A Method for Assessing Plasma Membrane Function as a Reflection of Mechanically Induced Cell Loading
Chapter 33. Cell Surface Biotinylation and Other Techniques for Determination of Surface Polarity of Epithelial Monolayers
Section 6: Mitochondria
Chapter 34. Protein Translocation into Mitochondria
Chapter 35. Polarographic Assays of Mitochondrial Functions
Section 7: Nuclear Transport
Chapter 36. Analysis of Nuclear Protein Import and Export in Digitonin-Permeabilized Cells
Chapter 37. Heterokaryons: An Assay for Nucleocytoplasmic Shuttling
Section 8: Chromatin Assembly
Chapter 38. DNA Replication-Dependent Chromatin Assembly System
Section 9: Signal Transduction Assays
Chapter 39. Cygnets: Intracellular Guanosine 3', 5'-Cyclic Monophosphate Sensing in Primary Cells Using Fluorescence Energy Transfer
Chapter 40. Ca2+ as a Second Messenger: New Reporters for Calcium (Cameleons and Camgaroos)
Chapter 41. Ratiometric Pericam
Chapter 42. Fluorescent Indicators for Imaging Protein Phosphorylation in Single Living Cells
Chapter 43. In Situ Electroporation of Radioactive Nucleotides: Assessment of Ras Activity or 32P Labeling of Cellular Proteins
Chapter 44. Dissecting Pathways; in Situ Electroporation for the Study of Signal Transduction and Gap Junctional Communication
Chapter 45. Detection of Protein-Protein Interactions in vivo Using Cyan and Yellow Fluorescent Proteins
Chapter 46. Tracking Individual Chromosomes with Integrated Arrays of lacop Sites and GFP-laci Repressor: Analyzing Position and Dynamics of Chromosomal Loci in Saccharomyces cerevisiae
Section 10: Assays and Models of in vitro and in vivo Motility
Chapter 47. Microtubule Motility Assays
Chapter 48. In vitro Assays for Mitotic Spindle Assembly and Function
Chapter 49. In vitro Motility Assays with Actin
Chapter 50. Use of Brain Cytosolic Extracts for Studying Actin-Based Motility of Listeria
Chapter 51. Pedestal Formation by Pathogenic Escherichia coli: A Model System for Studying Signal Transduction to the Actin Cytoskeleton
Chapter 52. Listeria monocytogenes: Techniques to Analyze Bacterial Infection in vitro
Section 11: Mechanical Stress in Single Cells
Chapter 53. Measurement of Cellular Contractile Forces Using Patterned Elastomer
Part C. APPENDIX
Chapter 54. Resources
CONTENTS OF VOLUME 3
PART A. Imaging Techniques
Section 1. Light Microscopy
Chapter 1. Fluoescence Microscopy
Chapter 2. Total Internal Reflection Flurescent Microscopy
Chapter 3. Band Limit and Appropriate Sampling in Microscopy
Chapter 4. Optical Tweezers: Application to the Study of Motor Proteins
Section 2. Digital Video Microscopy
Chapter 5. An Introduction to Electronic Image Acquisition during Light Microscopic Observation of Biological Specimens
Chapter 6. Video-Enhanced Contrast Microscopy
Section 3. Confocal Microscopy of Living Cells and Fixed Cells
Chapter 7. Spinning Disc Confocal Microscopy of Living Cells
Chapter 8. Confocal Microscopy of Drosophilia Embryos
Chapter 9. Ultraviolet Laser Microbeam for Dissection of Drosophilia Embryos
Section 4. Fluorescent Microscopy of Living Cells
Chapter 10. Introduction to Fluorescence Imaging of Live Cells: An Annotated Checklist
Chapter 11. Cytoskeleton Proteins
Chapter 12. Systematic Subcellular Localization of Novel Proteins
Chapter 13. Single Molecule Imaging in Living Cells by Total Internal Reflection Fluorescence Microscopy
Chapter 14. Live-Cell Fluorescent Speckle Microscopy of Actin Cytoskeletal Dynamic and Their Perturbation by Drug Perfusion
Chapter 15. Imaging Fluorescence Resonance Energy Transfer Between Green Fluorescent Protein Variants in Live Cells
Section 5. Use of Fluorescent Dyes for Studies of Intracellular Physiological Parameters
Chapter 16. Measurements of Endosomal pH in Live Cells by Dual-Excitation Fluorescence Imaging
Chapter 17. Genome-Wide Screening of Intracellular Protein Localization in Fission Yeast
Chapter 18. Large-Scale Protein Localization in Yeast
Section 6. Digital Image Processing, Analysis, Storage, and Display
Chapter 19. Lifting the Fog: Image Restoration by Deconvolution
Chapter 20. The State of teh Art in Biological Image Analysis
Chapter 21. Publishing and Finding Images in the BioImage Database, and Image Database for Biologists
PART B. Electron Microscopy
Section 7: Specimen Preparation Techniques
Chapter 22. Fixation and Embedding of Cells and Tissues for Transmission Electron Microscopy
Chapter 23. Negative Staining
Chapter 24. Glycerol Spraying/ Low-Angle Rotary Metal Shadowing
Section 8: Cryotechniques
Chapter 25. Rapid Freezing of Biological Specimens for Freeze Fracture and Deep Etching
Chapter 26. Freeze Fracture and Freeze Etching
Section 9: Electron Microscopy Studies of the Cytoskeleton
Chapter 27. Electron Microscopy of Extracted Cytoskeletons: Negative Staining, Cryoelectron Microscopy, and Correlation with Light Microscopy
Chapter 28. Correlative Light and Electron Microscopy of the Cytoskeleton
Section 10: Immunoelectron Microscopy
Chapter 29. Immunoelectron Microscopy with Lowicry Resins
Chapter 30. Use of Ultrathin Cryo- and Plastic Sections for Immunocytochemistry
Chapter 31. Direct Immunogold Labeling of Components within Protein Complexes
PART C. Scanning Probe and Scanning Electron Microscopy
Section 11: Scanning Probe and Scanning Electron Microscopy
Chapter 32. Atomic Force Microscopy in Biology
Chapter 33. Field Emission Scanning Electron Microscopy and Visualization of the Cell Interior
PART D. Microdissection
Section 12: Tissue and Chromosome Microdissection
Chapter 34. Laser Capture Microdissection
Chapter 35. Chromosome Microdissection Using Conventional Methods
Chapter 36. Micromanipulation of Chromosomes and the Mitotic Spindle Using Laser Microsurgery (Laser Scissors) and Laser-Induced Optical Forces (Laser Tweezers)
PART E. Tissue Arrays
Section 13: Tissue Arrays
Chapter 37. Tissue Microarrays
PART F. Cytogenetics and In Situ Hybridization
Section 14: Cytogenetics
Chapter 38. Basic Cytogenetic Techniques: Culturing, Slide Making, and G Banding
Chapter 39. A General and Reliable Method for Obtaining High-Yield Metaphasic Preparations from Adherent Cell Lines: Rapid Verification of Cell Chromosomal Content
Section 15: In Situ Hybridization
Chapter 40. Mapping Cloned DNA on Metaphase Chromosomes Using Fluorescence in Situ Hybridization
Chapter 41. Human Genome Project Resources for Breakpoint Mapping
Chapter 42. Fine Mapping of Gene Ordering by Elongated Chromosome Methods
Chapter 43. In Situ Hybridization Applicable to mRNA Species in Cultured Cells
Chapter 44. In Situ Hybridization for Simultaneous Detection of DNA, RNA, and Protein
Chapter 45. Fluorescent Visualization of Genomic Structure and DNA Replication at the Single Molecule Level
PART G. GENOMICS
Section 16: Genomics
Chapter 46. Genomic DNA Microarray for Comparative Genomic Hybridization
Chapter 47. Genotyping of Single Nucleotide Polymorphisms by Minisequencing Using Tag Arrays
Chapter 48. Single Nucleotide Polymorphism Analysis by Matrix-Associated Laser Desorption/ Ionization Time-of-Flight Mass Spectrometry
Chapter 49. Single Nucleotide Polymorphism Analysis by ZipCode-Tagged Microspheres
Chapter 50. Polymerase Chain Reaction-Based Amplification Method of Retaining the Quantitative Difference between Two Complex Genomes
PART H. Transgenic Knockouts, and Knockdown Methods
Section 17: Transgenic Knockouts, and Knockdown Methods
Chapter 51. Production of Transgenic Mice by Pronuclear Microinjection
Chapter 52. Gene Targeting by Homologous Recombination in Embryonic Stem Cells
Chapter 53. Conditional Knockouts: Cre-lox Systems
Chapter 54. RNAi-Mediated Gene Silencing in Mammalian Cells
Chapter 55. Antisense Oligonucleotides
CONTENTS OF VOLUME 4
PART A. Transfer of Macromolecules
Section 1: Proteins
Chapter 1. Impact-Mediated Cytoplasmic Loading of Macromolecules into Adherent Cells
Chapter 2. A Peptide Carrier for the Delivery of Biologicallly Active Proteins into Mammalian Cells: Application to the Delivery of Antibodies and Therapeutic Proteins
Chapter 3. Selective Permeabilization of the Cell-Surface Membrane by Streptolysin O
Section 2: Genes
Chapter 4. New Cationic Liposomes for Gene Ttransfer
Chapter 5. Cationic Polymers for Gene Delivery: Formation of Polycation-DNA Complexes and in vitro Transfection
Chapter 6. Electroporation of Living Embryos
Section 3: Somatic Cell Nuclear Transfer
Chapter 7. Somatic Cell Nuclear Transplantation
PART B. Expression Systems
Section 4: Expression Systems
Chapter 8. Expression of cDNA in Yeast
Chapter 9. Semliki Forest Virus Expression System
Chapter 10. Transient Expression of cDNAs in COS-1 Cells: Protein Analysis by Two-Dimensional Gel Electrophoresis
Chapter 11. High-Throughput Purification of Proteins from Escherichia coli
PART C. Gene Expression Profiling
Section 5: Differential Gene Expression
Chapter 12. Microarrays for Gene Expression Profiling: Fabrication of Oligonucleotide Microarrays, Isolation of RNA, Fluorescent Labelingof cRNA, Hybridization, and Scanning
Chapter 13. Array Express: A Public Repository for Microarray Data
Chapter 14. Serial Analysis of Gene Expression (SAGE): Detailed Protocol for Generating SAGE Catalogs of Mammalian Cell Transcriptomes
Chapter 15. Representational Difference Analysis: A Methodology to Study Differential Gene Expression
Chapter 16. Single Cell Gene Expression Profiling: Multiplexed Expression Fluorescence in situ Hybridization: Application to the Analysis of Cultured Cells
PART D. Proteins
Section 6: Protein Determination and Analysis
Chapter 17. Protein Determination
Chapter 18. Phosphopeptide Mapping: A Basic Protocol
Chapter 19. Coupling of Fluorescent Tags to Proteins
Chapter 20. Radioiodination of Proteins and Peptides
Section 7: Sample Fractionation for Proteomics
Chapter 21. Free-Flow Electrophoresis
Section 8: Gel Electrophoresis
Chapter 22. Gel-Based Proteomics: High-Resolution Two-Dimensional Gel Electrophoresis of Proteins, Isoelectric Focusing and Nonequilibrium pH Gradient Electrophoresis
Chapter 23. High Resolution Two-Dimensional Electrophoresis with Immobilized pH Gradients for Proteome Analysis
Chapter 24. Two-Dimensional Difference Gel Electrophoresis: Application for the Analysis of Differential Protein Expression in Multiple Biological Samples
Chapter 25. Affinity Electrophoresis for Studies of Biospecific Interactions: High-Resolution Two-Dimensinoal Affinity Electrophoresis for Separation of Hapten-Specific Polyclonal Antibodies in Murine Blood Plasma
Chapter 26. Image Analysis and Quantitation
Section 9: Detection of Proteins in Gels
Chapter 27. Protein Detection in Gels by Silver Staining: A Procedure Compatible with Mass Spectrometry
Chapter 28. Fluorescence Detection of Proteins in Gels Using SYPRO Dyes
Chapter 29. Autoradiography and Fluorography: Film-Based Techniques for Imaging Radioactivity in Flat Samples
Section 10: Gel Profiling of Posttranslationally Modified Proteins
Chapter 30. Two-Dimensional Gel Profiling of Posttranslationally Modified Proteins by in vivo Isotope Labeling
Section 11: Protein/ Protein and Protein/ Small Molecule Interactions
Chapter 31. Immunoprecipitation of Proteins Under Nondenaturing Conditions
Chapter 32. Nondenaturing Polyacrylamide Gel Electrophoresis as a Method for Studying Protein Interactions: Applications in the Analysis of Mitochondrial Oxidative Phosphorylation Complexes
Chapter 33. Affinity Purification with Natural Immobilized Ligands
Chapter 34. Analysis of Protein-Protein Interactions by Chemical Cross-Linking
Chapter 35. Peroxisomal Targeting as a Tool to Assess Protein-Protein Interactions
Chapter 36. Bimolecular Interaction Analysis Mass Spectroscopy
Chapter 37. Blot Overlays with 32P-Labeled GST-Ras Fusion Proteins: Application to Mapping Protein-Protein Interaction Slides
Chapter 38. Ligand Blot Overlay Assay: Detection of Ca+2-and Small GTP-Binding Proteins
Chapter 39. Modular Scale Yeast Two-Hybrid Screening
Section 12: Functional Proteomics
Chapter 40. Chromophore-Assisted Laser Inactivation of Proteins by Antibodies Labeled with Malachite Green
Section 13: Protein/ DNA Interactions
Chapter 41. Chromatin Immunoprecipitation (ChIP)
Chapter 42. Gel Mobility Shift Assay
Chapter 43. DNA Affinity Chromatography of Transcription Factors: The Oligonucleotide Trapping Approach
Section 14: Protein Degradation
Chapter 44. Protein Degradation Methods: Chaperone-Mediated Autophagy
Chapter 45. Methods in Protein Ubiquitination
Section 15: Mass Spectrometry: Protein Identification and Interactions
Chapter 46. Protein Identification and Sequencing by Mass Spectrometry
Chapter 47. Proteome Specific Sample Preparation Methods for Matrix-Assisted Laser Desorption/ Ionization Mass Spectrometry
Chapter 48. In-Gel Digestion of Protein Spots for Mass Spectrometry
Chapter 49. Peptide Sequencing by Tandem Mass Spectrometry
Chapter 50. Direct Database Searching Using Tandem Mass Spectra of Peptides
Chapter 51. Identification of Proteins from Organisms with Unsequenced Genomes by Tandem Mass Spectrometry and Sequence-Similarity Database Searching Tools
Chapter 52. Identification of Protein Phosphorylation Sites by Mass Spectrometry
Chapter 53. Analysis of Carbohydrates/ Glycoproteins by Mass Spectrometry
Chapter 54. Stable Isotope Labeling by Amino Acids in Cell Culture for Quantitative Proteomics
Chapter 55. Site-Specific, Stable Isotopic Labeling of Cysteinyl Peptides in Complex Peptide Mixtures
Chapter 56. Protein Hydrogen Exchange Measured by Electrospray Ionization Mass Spectrometry
Chapter 57. Nongel Based Proteomics: Selective Reversed-Phase Chromatographic Isolation of Methionine-Containing Peptides from Complex Peptide Mixtures
Chapter 58. Mass Spectrometry in Noncovalent Protein Interactions and Protein Assemblies
PART E. APPENDIX
Chapter 59. Bioinformatic Resources for in Silico Proteome Analysis
LIST OF SUPPLIERS
INDEX
INDEX
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